Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel). Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use in gel electrophoresis and Western blotting.Features of Spectra Multicolor Broad Range Pr. Hi, I encountered something strange the other day with my western blot: after transfer, the pre-stained ladder (bio-rad) showed up fine, but there was no protein whatsoever on the blot stained with Ponceau. SuperSignal West Femto Maximum Sensitivity Substrate. After transferring the protein bands onto the blot paper, stain the paper with Ponceau-S. Ladder but no protein - (Aug/24/2009 ) Ladder but no protein. Western blotting: detection of the nine unstained bands via the detection method used for the target protein. If you want to do with unstained ladder yet, I recommend to do all steps of western blot just routinely do.The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › And so, for this prestained protein marker, it has a molecular weight range from 10 to 180kDa. So, the first one is the standard one thats the bread and butter thatll work for a lot of different things. The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent). And lastly, you dont want to forget a molecular weight ladder, and so we sell a couple of these. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot. MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site.